abstract：:The mechanism by which macromolecular contents are concentrated during lymph transport was investigated in the collecting lymphatics of the mesentery of the rat. To examine changes in the concentration of lymph macromolecular tracer (fluorescein isothiocyanate-tagged dextran, MW 148,900) in the rhythmically contractil...

journal_title：Microvascular research

authors： Takahashi T,Shibata M,Kamiya A

更新日期：1997-11-01 00:00:00

abstract：:The large secreted glycoprotein thrombospondin-1 is a potent inhibitor of neovascularization in vivo. In order to better understand its mechanism of action, we have determined the full range of deficits thrombospondin can impose on cultured capillary endothelial cells. Exogenously added thrombospondin-1 blocked the ab...

journal_title：Microvascular research

authors： Tolsma SS,Stack MS,Bouck N

更新日期：1997-07-01 00:00:00

abstract：:Although vasodilation is conventionally held to be the predominant microvascular response to a wound, there has been no previous attempt to actually quantitate skin blood flow within and in the neighborhood of wounds. In particular, there has been no differentiation between sites with primarily nutritive (NUTR) blood ...

journal_title：Microvascular research

authors： Rendell MS,Milliken BK,Finnegan MF,Finney DA,Healy JC

更新日期：1997-05-01 00:00:00

abstract：:Mesangium enlargement is a central feature of diabetic nephropathy and almost certainly plays a pathogenic role in this condition. Previous studies have shown that mesangium degradation is reduced in a high glucose mileau. Plasmin has been shown to play an important role in extracellular matrix degradation, both direc...

journal_title：Microvascular research

authors： Fisher EJ,McLennan SV,Yue DK,Turtle JR

更新日期：1997-03-01 00:00:00

abstract：:We describe a sensitive, reliable, and convenient procedure to assay cell adhesion based on flow cytometry which allows quantitative studies of all major peripheral blood leukocytes adhering to microvascular endothelial cells. Using this assay, we show that adhesion of mononuclear cells is significantly increased in t...

journal_title：Microvascular research

authors： Heckmann M,Pirthauer M

更新日期：1996-09-01 00:00:00

abstract：:Previous findings have shown that pulmonary fibroblasts are associated with preexisting holes in the endothelial and epithelial basal laminae through which neutrophils appear to enter and leave the interstitium as they migrate from capillaries to alveoli. To determine their role in neutrophil migration, fibroblast org...

journal_title：Microvascular research

authors： Behzad AR,Chu F,Walker DC

更新日期：1996-05-01 00:00:00

abstract：:Immunological studies of human endothelial cells have been performed almost entirely on endothelial cells (EC) derived from foetal (umbilical vein) or adult (aorta) large vessels. Immunologically these EC have in common the fact that they are negative for major histocompatibility complex (MHC) class II antigens (HLA-D...

journal_title：Microvascular research

authors： McDouall RM,Yacoub M,Rose ML

更新日期：1996-03-01 00:00:00

abstract：:The objective of this study was to provide the first quantitative measurements of the process of vascular growth in the rat peritoneal disseminated tumor model in both arteriolar and venular systems. Male Fischer 344 rats were injected intraperitoneally with 1 x 10(7) cells of colon carcinoma cell line (RCN-9). Three ...

journal_title：Microvascular research

authors： Yanagi K,Ohshima N

更新日期：1996-01-01 00:00:00

abstract：:HPMECs were successfully isolated by differential trypsinization from peripheral lung lobes. The cells proliferated rapidly in EGM-MV with 10% FBS and were serially cultivated for more than 20 passages (1:4 split ratio) in vitro. Cells were characterized as endothelial based upon their cobblestone morphology, the pres...

journal_title：Microvascular research

authors： Shen J,Ham RG,Karmiol S

更新日期：1995-11-01 00:00:00

abstract：:Carrier-immobilized carbohydrates are valuable tools for assessing the glycoligand-binding capacity of cell surfaces. A panel of 10 types of fluorescent neoglycoproteins has been synthesized to determine the extent of their specific binding to endothelial cells in vitro that have been obtained from porcine brain, ovar...

journal_title：Microvascular research

authors： Plendl J,Sinowatz F,Auerbach R,Gabius HJ

更新日期：1995-09-01 00:00:00

abstract：:Neutrophil-derived hydrogen peroxide (H2O2) is believed to play an important role in inflammatory lung injury. We investigated the influence of pharmacological agents that increase intracellular c-AMP levels on endothelial and epithelial leakage in response to intravascular H2O2 challenge in buffer-perfused rabbit lun...

journal_title：Microvascular research

authors： Seeger W,Hansen T,Rössig R,Schmehl T,Schütte H,Krämer HJ,Walmrath D,Weissmann N,Grimminger F,Suttorp N

更新日期：1995-07-01 00:00:00

abstract：:We tested the hypothesis that cationic peptides (polyarginine; poly-argn) and eosinophil-derived major basic protein (MaBP) increase permeability by stimulating the release of histamine and/or nitric oxide. We used intravital microscopy, clearance, and integrated optical intensity (IOI), using FITC-dextran 150 (FITC-d...

journal_title：Microvascular research

authors： Minnicozzi M,Ramírez MM,Egan RW,Gleich GJ,Kobayashi I,Kim D,Durán WN

更新日期：1995-07-01 00:00:00

abstract：:Skeletal muscle arterioles are known to constrict upon elevation of ambient PO2. While several studies have shown that the endothelium plays an important role in this response, it is not clear how this response is mediated. We examined the oxygen-induced constriction of arterioles in the rat spinotrapezius muscle. Ele...

journal_title：Microvascular research

authors： Pries AR,Heide J,Ley K,Klotz KF,Gaehtgens P

更新日期：1995-05-01 00:00:00

abstract：:The effect of mean blood velocity on thrombus formation parameters was studied using mesenteric vessels of white rats. An advanced phenomenological theory, explaining the relationship between mean blood velocity and thrombus growth rate, is presented. The qualitative analysis of theoretical and experimental data have ...

journal_title：Microvascular research

authors： Petrishchev NN,Mikhailova IA

更新日期：1995-01-01 00:00:00

abstract：:An ex vivo perfused solid tumor preparation provides control over the physiological, biochemical, and pharmacological composition of the arterial input and easy access to the venous output. This is advantageous for studies of transport and metabolism in solid tumors. Here we present a tissue-isolated tumor preparation...

journal_title：Microvascular research

authors： Kristjansen PE,Roberge S,Lee I,Jain RK

更新日期：1994-11-01 00:00:00

abstract：:Despite intensive study in the area of angiogenesis, relatively little is known about normal angiogenesis in adult animals. Preliminary studies using the Griffonia simplicifolia I (GSI) lectin as a microvascular marker indicated that capillary sprouting occurs in the clear "windows" of normal intact adult rat mesenter...

journal_title：Microvascular research

authors： Hansen-Smith FM,Joswiak GR,Baustert JL

更新日期：1994-05-01 00:00:00

abstract：:Polymorphonuclear leukocytes are thought to play a pivotal role in the generation of ischemia-reperfusion-induced mucosal injury; however, their behavior in the intestinal microvasculature following this injury has not been directly examined. In this study intravital microscopy was used to investigate the dynamics of ...

journal_title：Microvascular research

authors： Boyd AJ,Sherman IA,Saibil FG

更新日期：1994-05-01 00:00:00

abstract：:Red blood cell (RBC)-plasma suspensions with a hematocrit of 10-12% were forced to flow through microchannels (equivalent diameter 6 microns, equivalent length 20 microns, 2600 in parallel) by applying a pressure difference of 20 cm H2O. Transit times for 100 microliters of fresh suspensions from 33 healthy male subje...

journal_title：Microvascular research

authors： Kikuchi Y,Da QW,Fujino T

更新日期：1994-03-01 00:00:00

abstract：:Electrophysiological characteristics of cultured human umbilical vein endothelial cells (HUVEC) were determined using the patch-clamp technique in the whole cell configuration. In isolated cells, membrane potential, capacitance, and input resistance were (Mean +/- SD) - 16.3 +/- 12.7 mV, 53.9 +/- 26 pF, and 2.3 +/- 1....

journal_title：Microvascular research

authors： Vargas FF,Caviedes PF,Grant DS

更新日期：1994-03-01 00:00:00

abstract：:Previously reported cell-flow microchannels in a single-crystal silicon substrate (Microvasc. Res. 44, 226-240, 1992) have been modified, and flow behavior of blood cells is described using flow rate-time curves and video pictures. The principal structure (2600 identically sized channels in parallel) was retained to g...

journal_title：Microvascular research

authors： Kikuchi Y,Sato K,Mizuguchi Y

更新日期：1994-01-01 00:00:00

abstract：:We examined the influence of local cooling (-15 degrees C, 60 sec) on blood flow velocity in nailfold capillaries and on laser Doppler flux (LDF) at the fingertip in 12 patients with primary Raynaud's phenomenon (PRP) and 13 patients with secondary Raynaud's (SRP) in comparison with 10 health controls. Skin temperatur...

journal_title：Microvascular research

authors： Lütolf O,Chen D,Zehnder T,Mahler F

更新日期：1993-11-01 00:00:00

abstract：:The fluorescence ratio imaging technique is currently applied in vitro and in vivo to measure intracellular as well as extracellular pH. To date, however, no effort has been made to assess the technique's ability to detect spatial pH gradients in vitro or in vivo, where pH gradients are known to exist, in a controlled...

journal_title：Microvascular research

authors： Martin GR,Jain RK

更新日期：1993-09-01 00:00:00

abstract：:Methods currently available for the examination of the retinal vasculature of laboratory animals have significant drawbacks. Fluorescein angiography of rodent eyes is hampered by a poor view of the peripheral retina and difficulty in performing fundus photography. Methods of staining or filling retinal vessels are unr...

journal_title：Microvascular research

authors： D'Amato R,Wesolowski E,Smith LE

更新日期：1993-09-01 00:00:00

abstract：:The kidneys of 14 female Wistar rats from 5 days to 18 months postnatally have been perfusion-fixed to study the development of glomerular capillaries using design-based stereological techniques. This report uses a topological definition of a capillary structural unit which forms the basis for the estimation of the nu...

journal_title：Microvascular research

authors： Nyengaard JR

更新日期：1993-05-01 00:00:00

abstract：:Techniques of intravital microscopy were used to assess the effect of the nonsteroidal anti-inflammatory drugs (NSAIDs), indomethacin and ibuprofen, on the microcirculation. Hemodynamics in venules of the rat mesentery were studied in terms of vessel diameter, red blood cell velocity, and leukocyte-endothelium interac...

journal_title：Microvascular research

authors： Slater C,House SD

更新日期：1993-03-01 00:00:00

abstract：:We have developed a simplified technique for culturing human umbilical vein endothelial cells under shear flow conditions, using prefabricated glass microcapillary tubes ("microslides") with a well-defined rectangular cross section and good optical quality. These microslides have been incorporated into a controlled fl...

journal_title：Microvascular research

authors： Cooke BM,Usami S,Perry I,Nash GB

更新日期：1993-01-01 00:00:00

abstract：:The extravascular uptake for labeled albumin and IgG and the extravascular masses for endogenous albumin and IgG were measured in skin and skeletal muscle from anesthetized rabbits following 24 hr of intermittent plasmapheresis. An amount of protein equivalent to the total intravascular protein mass was removed. There...

journal_title：Microvascular research

authors： Wallace JR,Powers MR,Bell DR

更新日期：1992-11-01 00:00:00

abstract：:Oxygen tension was recorded with double-barreled microelectrodes in the retina of anesthetized cats and monkeys. The oxygen tension (PO2) was often observed to undergo spontaneous irregular fluctuations with peak-to-peak amplitudes of about 3 to as much as 20 mm Hg. These fluctuations were observed only in the vascula...

journal_title：Microvascular research

authors： Braun RD,Linsenmeier RA,Yancey CM

更新日期：1992-07-01 00:00:00

abstract：:The contractile responses of cultured rat and calf endothelial cells (EC), vascular smooth muscle cells (VSMC), and fibroblasts (FB) to vasoactive mediators (thrombin, serotonin, bradykinin, and histamine), forskolin, and cytochalasin B were compared. Cells were grown on a pliable silicone membrane, and contraction wa...

journal_title：Microvascular research

authors： Boswell CA,Majno G,Joris I,Ostrom KA

更新日期：1992-03-01 00:00:00

abstract：:We determined whether the solvent drag reflection coefficient (sigma f) for total proteins of a canine perfused left lower lung lobe (LLL) preparation decreases at elevated venous pressures (Pv). We found that sigma f (estimated using the hematocrit-protein technique) remained constant at all Pv's (30-95 mm Hg) evalua...

journal_title：Microvascular research

authors： Maron MB,Lang SA

更新日期：1992-01-01 00:00:00

abstract：:During diabetes in rats, the choroid of the eye shows increased permeability to albumin, basement membrane thickening, and decreased anionic charge sites on the abluminal surfaces of the choriocapillary microvessels. In other microvascular beds, permeability differences are correlated with differences in luminal membr...

journal_title：Microvascular research

authors： Caldwell RB,Fitzgerald ME

更新日期：1991-11-01 00:00:00

abstract：:A mathematical model of nonsteady-state fluid movement in the lung interstitium during hydrodynamic loading is evaluated. The model is based on experimental results obtained in isolated perfused canine lungs. The interstitium is treated as a porous phase with an elastic framework, able to relax; all resistance to flux...

journal_title：Microvascular research

authors： Serikov VB

更新日期：1991-07-01 00:00:00

abstract：:This study was undertaken to determine if coronary vascular permeability (CVP) increases and if myocardial edema develops in the canine heart after local exposure to histamine. Histamine (50 or 15 micrograms/min) or compound 48/80 (0.1-0.2 mg/kg) was infused into the left anterior descending coronary artery (LAD) of o...

journal_title：Microvascular research

authors： Pilati CF

更新日期：1991-05-01 00:00:00

abstract：:The therapeutic efficacy of various genetically engineered macromolecules is determined by their delivery and distribution in tumors. We have recently developed mathematical models which describe the interstitial velocity, pressure, and concentration profiles of macromolecules over the length scale of a solid tumor (B...

journal_title：Microvascular research

authors： Baxter LT,Jain RK

更新日期：1991-03-01 00:00:00

abstract：:A cytochemical study of vascular and cellular development in fetal adipose tissue was conducted utilizing 10 plant lectins (fluorescein isothiocyanate (FITC)-labeled), antibodies against laminin, types II and IV collagen, and a probe for actin. Throughout fetal development (50-110 days) blood vessels were stained by g...

journal_title：Microvascular research

authors： Hausman GJ,Wright JT,Thomas GB

更新日期：1991-01-01 00:00:00

abstract：:In this study cerebral capillary bed structure and the effects of chronic hypertension on these systems have been assessed in 6- to 7-month-old spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats. Capillary diameter (D), profile frequency (Na), volume fraction (Vv), and surface area (Sv) were quantitated by l...

journal_title：Microvascular research

authors： Lin SZ,Sposito N,Pettersen S,Rybacki L,McKenna E,Pettigrew K,Fenstermacher J

更新日期：1990-11-01 00:00:00

abstract：:The microcirculation in normal and arthritic juxtaarticular bone was studied in 16 young dogs with carragheenan-induced arthritis of one knee. The regional blood flow was determined by the tissue uptake of intracardially injected 15-microns 141Ce-labeled microspheres, and the microvascular plasma volume was determined...

journal_title：Microvascular research

authors： Hansen ES,Søballe K,Kjølseth D,Henriksen TB,He SZ

更新日期：1990-09-01 00:00:00

abstract：:It is generally assumed that when a muscle is shortened or extended the total length of capillaries does not change, implying that capillaries are nondistensible, longitudinally. On the basis of stereological estimates of capillary anisotropy versus sarcomere length, we propose that as long as capillaries are in a tor...

journal_title：Microvascular research

authors： Ellis CG,Mathieu-Costello O,Potter RF,MacDonald IC,Groom AC

更新日期：1990-07-01 00:00:00

abstract：:A simple model has been developed for simulation of oxygen transport to and from blood flowing in conduits of the diameter of arterioles and larger (greater than or equal to 20 microns). The basis is the large capillary model (LCAP) of P.K. Nair, et al. 1989 which has been validated experimentally. Detailed calculatio...

journal_title：Microvascular research

authors： Nair PK,Huang NS,Hellums JD,Olson JS

更新日期：1990-03-01 00:00:00

abstract：:The purpose of the present study was to investigate the nervous control of gingival blood flow in cats. Gingival blood flow was measured by laser Doppler flowmeter in 75 cats during electrical stimulation and cutting or ligation of the inferior alveolar nerve and cervical sympathetic nerve without sympathectomy or pre...

journal_title：Microvascular research

authors： Izumi H,Kuriwada S,Karita K,Sasano T,Sanjo D

更新日期：1990-01-01 00:00:00